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Proteomics in cardiovascular research
Lasse Lorentzen
21/4-2022
Proteomics as a tool for precision medicine
Precision medicine
Proteomics of carotid artery plaques
Differentially regulated proteins
Clustering of patient samples
Over-representation analysis
Looking at proteins are not enough
More than 1 million functional proteoforms
Protein function can be altered by post-translationally
Proteolytic degradation can alter plaque stability in atherosclerosis
Proteomics workflow
Differentially regulated free N-termini
Clustering of patient samples
Degradation of Collagens in soft lesions
Summary
Proteomics is a powerful technique to study processes associated with plaque destabilization
Proteome profiles can distinguish hard and soft plaques
Also hard and mixed plaques
N-terminal proteomics can better distinguish plaques of different morphology
Next
Correlate proteomics data with contrast-enhanced ultrasound
Identify circulating biomarkers of plaque destablization
Use a similar approach to study aneurysms
Study other post-translational modifications and their association with atherosclerosis and plaque destablization
Protein function can be altered by post-translationally
Thanks
Prof. Jonas Eiberg, Karin Yeung, Prof. Henrik Sillesen
Prof. Michael Davies
Christine Chuang
Protein Oxidation Group
Thank you for listening