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Proteomics in cardiovascular research

Lasse Lorentzen

21/4-2022

Proteomics as a tool for precision medicine

Precision medicine

Proteomics of carotid artery plaques

Differentially regulated proteins

Clustering of patient samples

Over-representation analysis

Looking at proteins are not enough

More than 1 million functional proteoforms

Protein function can be altered by post-translationally

Proteolytic degradation can alter plaque stability in atherosclerosis

Proteomics workflow

Differentially regulated free N-termini

Clustering of patient samples

Degradation of Collagens in soft lesions

Summary

  • Proteomics is a powerful technique to study processes associated with plaque destabilization
  • Proteome profiles can distinguish hard and soft plaques
    • Also hard and mixed plaques
  • N-terminal proteomics can better distinguish plaques of different morphology

Next

  • Correlate proteomics data with contrast-enhanced ultrasound
  • Identify circulating biomarkers of plaque destablization
  • Use a similar approach to study aneurysms
  • Study other post-translational modifications and their association with atherosclerosis and plaque destablization

Protein function can be altered by post-translationally

Thanks

  • Prof. Jonas Eiberg, Karin Yeung, Prof. Henrik Sillesen
  • Prof. Michael Davies
  • Christine Chuang
  • Protein Oxidation Group

Thank you for listening